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rox reference dye  (TaKaRa)


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    Structured Review

    TaKaRa rox reference dye
    Rox Reference Dye, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 50250 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rox+dye+ii/TB+Green+Premix+Ex+Taq+II/pm41698644-79-22-32
    Average 99 stars, based on 50250 article reviews
    rox reference dye - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: Dietary Green-Algae Chaetomorpha linum Extract Supplementation on Growth, Digestive Enzymes, Antioxidant Defenses, Immunity, Immune-Related Gene Expression, and Resistance to Aeromonas hydrophila in Adult Freshwater Snail, Bellamya bengalensis .
    Article Snippet: PCR assays were conducted using a SimpliAmp Thermal Cycler, Thermo Fisher Scientific (Model No: A24812, Waltham, MA, USA) System with a reaction volume of 20 μL. .. This volume comprised 10 μL of Taq II (Takara Biotechnology), 0.5 μL each of forward and reverse PCR primers (10 μM), 2.5 μL of cDNA template, 0.5 μL of ROX Dye II (Takara), https://doi.org/10.3390/ani16020289 and 6.5 μL of RNase-free water. ..

    Article Title: Estradiol enhanced neuronal plasticity and ameliorated astrogliosis in human iPSC-derived neural models
    Article Snippet: RNA from the cultured cells was extracted using the RNeasy Mini Kit (Qiagen). cDNA was synthesized from the extracted RNA using the iScript cDNA synthesis kit (Bio-Rad). .. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) was performed using 4 ng/mL cDNA, TB Green II, and Rox Dye II (TaKaRa) with 20 mM of each primer. .. The amplificationwas carried out using a ViiA 7 Real-Time PCR System (Thermo Fisher Scientific 4453723) according to the manufacturer's instructions.

    Article Title: Establishment of KEIOi005-A iPSC line from urine-derived cells (UDCs) of a mild Alzheimer's disease (AD) donor with multiple risk SNPs for sporadic Alzheimer's disease (sAD).
    Article Snippet: RNA was extracted using the RNeasy Mini Kit (Qiagen), and cDNA was synthesized using the iScript cDNA synthesis kit (Bio-Rad). .. Quantitative reverse transcription polymerase chain reaction (RT-qPCR) was performed using 4 ng/μL cDNA, TB Green II, and Rox Dye II (TaKaRa) with 20 μM of each primer using a ViiA 7 Real-Time PCR System (Thermo Fisher Scientific 4453723) (Table 2). ..

    Amplification:

    Article Title: Fungal Integrated Histomolecular Diagnosis Using Targeted Next-Generation Sequencing on Formalin-Fixed Paraffin-Embedded Tissues.
    Article Snippet: Histopathology is the gold standard for fungal infection (FI) diagnosis, but it does not provide a genus and/or species identification.. The objective of the present study was to develop targeted next-generation sequencing (NGS) on formalinfixed tissue samples (FTs) to achieve a fungal integrated histomolecular diagnosis.. Nucleic acid extraction was optimized on a first group of 30 FTs with Aspergillus fumigatus or Mucorales infection by macrodissecting the microscopically identified fungalrich area and comparing Qiagen and Promega extraction methods through DNA amplification by A. fumigatus and Mucorales primers.

    Article Title: A human induced pluripotent stem cell model from a patient with hereditary cerebral small vessel disease carrying a heterozygous R302Q mutation in HTRA1
    Article Snippet: RNA was extracted using the RNeasy Micro Kit (Qiagen, Venlo, Netherlands), and cDNA was synthesized using iScript cDNA Synthesis Kit with DNase I treatment (Bio-Rad, CA, USA), which were performed according to manufacturers’ instructions. .. 4 ng of cDNA diluted in water was amplified with 20 μM forward and reverse primers (summarized in Supplementary Table S ), TB Green II, and ROX Dye II (Takara Bio, Shiga, Japan) for quantitative PCR by ViiA7 Real-Time PCR system (Thermo Fisher Scientific, MA, USA). ..

    Real-time Polymerase Chain Reaction:

    Article Title: Molecular identification and functional characterization of a WAP domain-containing protein in Apostichopus japonicus
    Article Snippet: WAP domain-containing proteins are important immune effectors that play vital roles in regulating host immune response, such as anti-microbial invasion and tissue repair at mucosal surfaces.. In our study, the whole-length cDNA of a protein with a single WAP domain was cloned from Apostichopus japonicus (termed AjWFDC-like), and its roles in immune defense were further investigated.. The AjWFDC-like protein shared a 17 aa signal peptide, a typical WAP domain (23–69 aa), a KAZAL domain (76–22 aa), and a thyroglobulin type I domain (156 aa-204 aa).

    Article Title: A human induced pluripotent stem cell model from a patient with hereditary cerebral small vessel disease carrying a heterozygous R302Q mutation in HTRA1
    Article Snippet: RNA was extracted using the RNeasy Micro Kit (Qiagen, Venlo, Netherlands), and cDNA was synthesized using iScript cDNA Synthesis Kit with DNase I treatment (Bio-Rad, CA, USA), which were performed according to manufacturers’ instructions. .. 4 ng of cDNA diluted in water was amplified with 20 μM forward and reverse primers (summarized in Supplementary Table S ), TB Green II, and ROX Dye II (Takara Bio, Shiga, Japan) for quantitative PCR by ViiA7 Real-Time PCR system (Thermo Fisher Scientific, MA, USA). ..

    Article Title: Establishment and preliminary application of duplex fluorescence quantitative PCR for porcine circoviruses type 2 and type 3
    Article Snippet: .. The final real-time PCR system was as follows: the reaction system contained 10 μL of 2 × Premix Ex Taq (Takara, Dalian, China), 0.4 μL of primers (10 μmol/L), 0.4 μL of probes (10 μmol/L), 2 μL of template DNA, and 0.2 μL of ROX Dye II (Takara, Dalian, China), with sterile water added to obtain a final volume of 20 μL. ..

    Article Title: Establishment of KEIOi005-A iPSC line from urine-derived cells (UDCs) of a mild Alzheimer's disease (AD) donor with multiple risk SNPs for sporadic Alzheimer's disease (sAD).
    Article Snippet: RNA was extracted using the RNeasy Mini Kit (Qiagen), and cDNA was synthesized using the iScript cDNA synthesis kit (Bio-Rad). .. Quantitative reverse transcription polymerase chain reaction (RT-qPCR) was performed using 4 ng/μL cDNA, TB Green II, and Rox Dye II (TaKaRa) with 20 μM of each primer using a ViiA 7 Real-Time PCR System (Thermo Fisher Scientific 4453723) (Table 2). ..

    SYBR Green Assay:

    Article Title: Molecular identification and functional characterization of a WAP domain-containing protein in Apostichopus japonicus
    Article Snippet: WAP domain-containing proteins are important immune effectors that play vital roles in regulating host immune response, such as anti-microbial invasion and tissue repair at mucosal surfaces.. In our study, the whole-length cDNA of a protein with a single WAP domain was cloned from Apostichopus japonicus (termed AjWFDC-like), and its roles in immune defense were further investigated.. The AjWFDC-like protein shared a 17 aa signal peptide, a typical WAP domain (23–69 aa), a KAZAL domain (76–22 aa), and a thyroglobulin type I domain (156 aa-204 aa).

    Sterility:

    Article Title: Establishment and preliminary application of duplex fluorescence quantitative PCR for porcine circoviruses type 2 and type 3
    Article Snippet: .. The final real-time PCR system was as follows: the reaction system contained 10 μL of 2 × Premix Ex Taq (Takara, Dalian, China), 0.4 μL of primers (10 μmol/L), 0.4 μL of probes (10 μmol/L), 2 μL of template DNA, and 0.2 μL of ROX Dye II (Takara, Dalian, China), with sterile water added to obtain a final volume of 20 μL. ..

    Extraction:

    Article Title: Fungal Integrated Histomolecular Diagnosis Using Targeted Next-Generation Sequencing on Formalin-Fixed Paraffin-Embedded Tissues.
    Article Snippet: Histopathology is the gold standard for fungal infection (FI) diagnosis, but it does not provide a genus and/or species identification.. The objective of the present study was to develop targeted next-generation sequencing (NGS) on formalinfixed tissue samples (FTs) to achieve a fungal integrated histomolecular diagnosis.. Nucleic acid extraction was optimized on a first group of 30 FTs with Aspergillus fumigatus or Mucorales infection by macrodissecting the microscopically identified fungalrich area and comparing Qiagen and Promega extraction methods through DNA amplification by A. fumigatus and Mucorales primers.

    Inhibition:

    Article Title: Fungal Integrated Histomolecular Diagnosis Using Targeted Next-Generation Sequencing on Formalin-Fixed Paraffin-Embedded Tissues.
    Article Snippet: Histopathology is the gold standard for fungal infection (FI) diagnosis, but it does not provide a genus and/or species identification.. The objective of the present study was to develop targeted next-generation sequencing (NGS) on formalinfixed tissue samples (FTs) to achieve a fungal integrated histomolecular diagnosis.. Nucleic acid extraction was optimized on a first group of 30 FTs with Aspergillus fumigatus or Mucorales infection by macrodissecting the microscopically identified fungalrich area and comparing Qiagen and Promega extraction methods through DNA amplification by A. fumigatus and Mucorales primers.

    Control:

    Article Title: Fungal Integrated Histomolecular Diagnosis Using Targeted Next-Generation Sequencing on Formalin-Fixed Paraffin-Embedded Tissues.
    Article Snippet: Histopathology is the gold standard for fungal infection (FI) diagnosis, but it does not provide a genus and/or species identification.. The objective of the present study was to develop targeted next-generation sequencing (NGS) on formalinfixed tissue samples (FTs) to achieve a fungal integrated histomolecular diagnosis.. Nucleic acid extraction was optimized on a first group of 30 FTs with Aspergillus fumigatus or Mucorales infection by macrodissecting the microscopically identified fungalrich area and comparing Qiagen and Promega extraction methods through DNA amplification by A. fumigatus and Mucorales primers.

    Reverse Transcription:

    Article Title: Establishment of KEIOi005-A iPSC line from urine-derived cells (UDCs) of a mild Alzheimer's disease (AD) donor with multiple risk SNPs for sporadic Alzheimer's disease (sAD).
    Article Snippet: RNA was extracted using the RNeasy Mini Kit (Qiagen), and cDNA was synthesized using the iScript cDNA synthesis kit (Bio-Rad). .. Quantitative reverse transcription polymerase chain reaction (RT-qPCR) was performed using 4 ng/μL cDNA, TB Green II, and Rox Dye II (TaKaRa) with 20 μM of each primer using a ViiA 7 Real-Time PCR System (Thermo Fisher Scientific 4453723) (Table 2). ..



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